Information-enriched characterization of cell populations. Morphological info from photographs can incorporate to the richness of flow cytometry info. One example is, the figure displays an or else standard apoptosis assay utilizing Annexin V and PI, introducing cell imaging to characterize cells in Each individual populace to expose morphologically unique features. These insights couldn't are actually attained from multiplex staining by itself.
Whether you’re a rookie or an authority, explore our sources to progress your analysis and experience in flow cytometry.
Merge acoustic focusing technologies with superior-parameter Assessment for versatile spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing that has a high-speed brightfield digital camera for simultaneous large throughput flow cytometry and higher resolution brightfield imaging.
"A picture isn't really fairly well worth one thousand dots, however it actually helps you to see photos. It is excellent to see photos that correlate with staining. For instance, AnnexinV-positive cells which have been morphologically various than balanced cells, or CD14+ monocytes which can be larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a skilled user has gated singlets confidently. Following assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has greater than four% aggregates.
Optimizing gating system. Even robust manual singlet gating is error-prone and remains a subjective choice point in Practically all flow cytometry assays. Imaging can be utilized to confirm and regulate gates to incorporate only solitary cells of desire.
Our experts will style and design panels in your case as often times you would like—totally free, no product buy necessary.
Early signs of this contamination had originally been dismissed as debris. Backgating to discover new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also means that you can use morphological options to find intriguing subpopulations that would not be obvious from flow cytometry info on your own.
Consumers can see occasion pictures in serious-time to confirm sample good quality, enhance protocols, and file the morphology of every cell populace identified for Examination. Imaging capture also Gains from acoustic concentrating, which really helps to situation the cells to ensure that a sharp, centered graphic is attained.
Sound waves enable precise mobile alignment, causing Remarkable info quality and ~10X speedier more info sample flow prices.
Application Take note: Automatic picture Investigation decreases user-to-user variability in flow cytometry gating techniques
Leverage the power of Highly developed technological innovation and innovation to speed up your discoveries. Believe in in the Attune Flow Cytometer spouse and children to deliver the trustworthiness, speed, and precision that set your get the job done aside.
No wash/no lyse leukocyte Assessment with imaging. Cells ended up obtained from 24-hour-old blood by dilution in one mM EDTA ( Professional UX/UI permits users to practice AI versions for expanded purposes. (A) The “Train Design” element presents annotation resources and genuine-time model schooling accuracy.
At the heart of each Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which utilizes seem waves to meticulously align cells inside the flow mobile. This ground breaking approach surpasses regular hydrodynamic strategies, causing speedier processing speeds, Excellent info top quality, and improved sample throughput.
The Attune Xenith cytometer is really a high-parameter spectral analyzer, giving both of those spectral unmixing and standard flow abilities for in depth and functional mobile Evaluation.
Agilent Benefit Assure assures you at least a decade of instrument use from your date you purchase. Otherwise, We're going to credit rating you Using the residual worth of the process toward an upgraded model.
What gave the impression to be a homogeneous populace is definitely more various—an insight that needs to be considered when interpreting final results.